{"id":2049,"date":"2020-10-29T13:07:08","date_gmt":"2020-10-29T13:07:08","guid":{"rendered":"http:\/\/onlineclassesguru.com\/?p=2049"},"modified":"2020-10-29T13:07:08","modified_gmt":"2020-10-29T13:07:08","slug":"lab-quiz","status":"publish","type":"post","link":"https:\/\/onlineclassesguru.com\/index.php\/2020\/10\/29\/lab-quiz\/","title":{"rendered":"lab quiz"},"content":{"rendered":"<style type=\"text\/css\"><\/style><p><strong>QUESTION 1<\/strong><\/p>\n<p>1. Most scientists who use DNA microarrays obtain them<\/p>\n<p>&nbsp;<\/p>\n<p>from \u00a0\u00a0companies that mass produce them<\/p>\n<p>&nbsp;<\/p>\n<p>from \u00a0\u00a0the government<\/p>\n<p>&nbsp;<\/p>\n<p>by \u00a0\u00a0making them in their labs<\/p>\n<p>&nbsp;<\/p>\n<p>by \u00a0\u00a0exploiting ambitious graduate students<\/p>\n<p><strong>QUESTION 2<\/strong><\/p>\n<p>1. &#8220;In this study, cancerous and normal skin tissue samples were excised with a scalpel. Each skin sample was then placed into a sample tube along with solvent, shaken on a vortex and centrifuged. When the sample tubes came out of the centrifuge, there was a top layer and a bottom layer. For our purposes, we went on to use ______________ for further processing.&#8221;<\/p>\n<p>&nbsp;<\/p>\n<p>only \u00a0\u00a0the top layer<\/p>\n<p>&nbsp;<\/p>\n<p>only \u00a0\u00a0the bottom layer<\/p>\n<p>&nbsp;<\/p>\n<p>a \u00a0\u00a0mixture of top and bottom layers<\/p>\n<p>&nbsp;<\/p>\n<p>none \u00a0\u00a0of the options listed<\/p>\n<p><strong>QUESTION 3<\/strong><\/p>\n<p>1. The purpose of the vortex instrument is to<\/p>\n<p>&nbsp;<\/p>\n<p>vigorously \u00a0\u00a0shake the tissue sample in the solvent so that it dissolves.<\/p>\n<p>&nbsp;<\/p>\n<p>spin \u00a0\u00a0the tissue\/solvent mixture around to separate the solids<\/p>\n<p>&nbsp;<\/p>\n<p>cause \u00a0\u00a0the mRNA to bind to the pellets<\/p>\n<p>&nbsp;<\/p>\n<p>dispose \u00a0\u00a0of biohazardous waste safely<\/p>\n<p><strong>QUESTION 4<\/strong><\/p>\n<p>1. &#8220;Once the RNA is isolated from the DNA, proteins and other materials, the solvent\/RNA mixture is run through a column of all beads. Each bead has several short strands of polyT RNA sticking out of it. In this step:&#8221;<\/p>\n<p>&nbsp;<\/p>\n<p>&#8220;mRNA \u00a0\u00a0sticks to the beads, while rRNA and tRNA run right through the column&#8221;<\/p>\n<p>&nbsp;<\/p>\n<p>&#8220;tRNA \u00a0\u00a0sticks to the beads, while mRNA and rRNA run right through the column&#8221;<\/p>\n<p>&nbsp;<\/p>\n<p>&#8220;rRNA \u00a0\u00a0and tRNA stick to the beads, while mRNA runs right through the column&#8221;<\/p>\n<p>&nbsp;<\/p>\n<p>none \u00a0\u00a0of the options listed<\/p>\n<p><strong>QUESTION 5<\/strong><\/p>\n<p>1. &#8220;Once the mRNA is isolated, you make a DNA copy of it using by adding polyT primers, labeled DNA nucleotides, and an enzyme known as:&#8221;<\/p>\n<p>&nbsp;<\/p>\n<p>reverse \u00a0\u00a0transcriptase<\/p>\n<p>&nbsp;<\/p>\n<p>tyrosine \u00a0\u00a0hydroxylase<\/p>\n<p>&nbsp;<\/p>\n<p>alcohol \u00a0\u00a0dehydrogenase<\/p>\n<p>&nbsp;<\/p>\n<p>ATP \u00a0\u00a0kinase<\/p>\n<p><strong>QUESTION 6<\/strong><\/p>\n<p>1. A DNA copy of a mRNA transcript is known as<\/p>\n<p>&nbsp;<\/p>\n<p>complementary \u00a0\u00a0DNA (abbreviated cDNA)<\/p>\n<p>&nbsp;<\/p>\n<p>copied \u00a0\u00a0DNA (abbreviated cDNA)<\/p>\n<p>&nbsp;<\/p>\n<p>mitochondrial \u00a0\u00a0DNA (abbreviated mtDNA)<\/p>\n<p>&nbsp;<\/p>\n<p>recombinant \u00a0\u00a0DNA (abbreviated rDNA)<\/p>\n<p><strong>QUESTION 7<\/strong><\/p>\n<p>1. Each spot on the DNA microarray in embedded with<\/p>\n<p>&nbsp;<\/p>\n<p>copies \u00a0\u00a0of DNA from one particular gene<\/p>\n<p>&nbsp;<\/p>\n<p>copies \u00a0\u00a0of DNA from several different genes with similar functions<\/p>\n<p>&nbsp;<\/p>\n<p>copies \u00a0\u00a0of DNA from several different genes with different functions<\/p>\n<p>&nbsp;<\/p>\n<p>copies \u00a0\u00a0of mRNA from one or more genes<\/p>\n<p><strong>QUESTION 8<\/strong><\/p>\n<p>1. &#8220;When we say that the cDNA derived from our sample has hybridized to a particular spot on the array, we mean that: &#8221;<\/p>\n<p>&nbsp;<\/p>\n<p>The \u00a0\u00a0cDNA from the sample has stuck to the DNA on the microarray at that point.<\/p>\n<p>&nbsp;<\/p>\n<p>The \u00a0\u00a0cDNA from the sample is jumbled and cannot be trusted.<\/p>\n<p>&nbsp;<\/p>\n<p>The \u00a0\u00a0cDNA has been used to make an mRNA copy<\/p>\n<p>&nbsp;<\/p>\n<p>You \u00a0\u00a0need to buy microarrays from different manufacturers and triangulate the \u00a0\u00a0results<\/p>\n<p><strong>QUESTION 9<\/strong><\/p>\n<p>1. &#8220;In this particular experiment, we used red-labeled DNA to process the sample from _____________ tissue and the green-labeled DNA to process the sample from ___________. &#8221;<\/p>\n<p>&nbsp;<\/p>\n<p>cancerous; \u00a0\u00a0normal<\/p>\n<p>&nbsp;<\/p>\n<p>normal; \u00a0\u00a0cancerous<\/p>\n<p>&nbsp;<\/p>\n<p>normal; \u00a0\u00a0normal<\/p>\n<p>&nbsp;<\/p>\n<p>cancerous; \u00a0\u00a0cancerous<\/p>\n<p><strong>QUESTION 10<\/strong><\/p>\n<p>1. &#8220;After scanning the green labeled areas and the red labeled areas, when we combine the two images, the spots that show up as yellow correspond to &#8221;<\/p>\n<p>&nbsp;<\/p>\n<p>genes \u00a0\u00a0expressed by both normal or cancerous skin cells.<\/p>\n<p>&nbsp;<\/p>\n<p>genes \u00a0\u00a0that were not expressed at all in either normal or cancerous skin cells<\/p>\n<p>&nbsp;<\/p>\n<p>&#8220;genes \u00a0\u00a0expressed by cancerous, but not normal skin cells&#8221;<\/p>\n<p>&nbsp;<\/p>\n<p>&#8220;genes \u00a0\u00a0expressed by normal, but not cancer skin cells. &#8221;<\/p>\n<p><strong>QUESTION 11<\/strong><\/p>\n<p>1. &#8220;In interpreting the results of this study, spots on the microarray that are red correspond to:&#8221;<\/p>\n<p>&nbsp;<\/p>\n<p>genes \u00a0\u00a0that are turned up by cancer<\/p>\n<p>&nbsp;<\/p>\n<p>genes \u00a0\u00a0that are turned down by cancer<\/p>\n<p>&nbsp;<\/p>\n<p>genes \u00a0\u00a0that are unaffected by cancer<\/p>\n<p>&nbsp;<\/p>\n<p>genes \u00a0\u00a0that aren t expressed in normal or cancerous cells<\/p>\n<p><strong>QUESTION 12<\/strong><\/p>\n<p>1. &#8220;In interpreting the results of this study, spots on the the microarray that are green correspond to genes that are &#8221;<\/p>\n<p>&nbsp;<\/p>\n<p>genes \u00a0\u00a0that are turned down by cancer<\/p>\n<p>&nbsp;<\/p>\n<p>genes \u00a0\u00a0that are turned up by cancer<\/p>\n<p>&nbsp;<\/p>\n<p>genes \u00a0\u00a0that are unaffected by cancer<\/p>\n<p>&nbsp;<\/p>\n<p>genes \u00a0\u00a0that aren t expressed in normal or cancerous cells<\/p>\n<p><strong>QUESTION 13<\/strong><\/p>\n<p>1. &#8220;When the DNA microarray study tells us that a large number of genes have been turned up (or turned down) by a disorder, the most likely explanation is that &#8221;<\/p>\n<p>&nbsp;<\/p>\n<p>the \u00a0\u00a0turned up genes are likely controlled by a gene that has gone bad<\/p>\n<p>&nbsp;<\/p>\n<p>all \u00a0\u00a0of these genes are genes that have gone bad themselves<\/p>\n<p>&nbsp;<\/p>\n<p>even \u00a0\u00a0just one mutation in any of these affected genes would have been sufficient \u00a0\u00a0to cause the disorder<\/p>\n<p>&nbsp;<\/p>\n<p>the \u00a0\u00a0cytoplasm has too many free radicals in it<\/p>\n<p><strong>QUESTION 14<\/strong><\/p>\n<p>1. A gene shown by the microarray to be expressed is :<\/p>\n<p>&nbsp;<\/p>\n<p>&#8220;probably \u00a0\u00a0making protein, but a protein expression analysis would be needed to know for \u00a0\u00a0sure&#8221;<\/p>\n<p>&nbsp;<\/p>\n<p>definitely \u00a0\u00a0making protein<\/p>\n<p>&nbsp;<\/p>\n<p>definitely \u00a0\u00a0not making protein<\/p>\n<p>&nbsp;<\/p>\n<p>probably \u00a0\u00a0making protein but there&#8217;s no way to know for sure<\/p>\n<p><strong>QUESTION 15<\/strong><\/p>\n<p>1. &#8220;In the application of this technique to skin cancer, a gene that has gone bad &#8221;<\/p>\n<p>&nbsp;<\/p>\n<p>&#8220;may \u00a0\u00a0or may not be identified at all, but the overall pattern of results can give \u00a0\u00a0important clues&#8221;<\/p>\n<p>&nbsp;<\/p>\n<p>can \u00a0\u00a0almost always be identified via DNA microarray<\/p>\n<p><center><a href=\"http:\/\/onlineclassesguru.com\/orders\/ordernow\"><img decoding=\"async\" src=\"https:\/\/encrypted-tbn0.gstatic.com\/images?q=tbn:ANd9GcTyj99p60XCLyLk1htB7-1neRt8-2QdnenNlQ&usqp=CAU\"target=\"_http:\/\/onlineclassesguru.com\/orders\/ordernow\"\/><\/center><p>","protected":false},"excerpt":{"rendered":"<p>QUESTION 1 1. Most scientists who use DNA microarrays obtain them &nbsp; from \u00a0\u00a0companies that mass produce them &nbsp; from \u00a0\u00a0the government &nbsp; by \u00a0\u00a0making them in their labs &nbsp; by \u00a0\u00a0exploiting ambitious graduate students QUESTION 2 1. &#8220;In this study, cancerous and normal skin tissue samples were excised with a scalpel. Each skin sample&#8230;<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-2049","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v17.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>lab quiz - onlineclassesguru<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/onlineclassesguru.com\/index.php\/2020\/10\/29\/lab-quiz\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"lab quiz - onlineclassesguru\" \/>\n<meta property=\"og:description\" content=\"QUESTION 1 1. 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