{"id":1890,"date":"2020-10-29T10:58:01","date_gmt":"2020-10-29T10:58:01","guid":{"rendered":"http:\/\/onlineclassesguru.com\/?p=1890"},"modified":"2020-10-29T10:58:01","modified_gmt":"2020-10-29T10:58:01","slug":"enzymes-2","status":"publish","type":"post","link":"https:\/\/onlineclassesguru.com\/index.php\/2020\/10\/29\/enzymes-2\/","title":{"rendered":"Enzymes"},"content":{"rendered":"<style type=\"text\/css\"><\/style><h2>INSTRUCTIONS:<\/h2>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h2>\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0On your own and without assistance, complete this\u00a0Lab 4\u00a0Answer Sheet\u00a0electronically and submit it via the\u00a0Assignments Folder\u00a0by the date listed in the\u00a0Course Schedule\u00a0(under\u00a0Syllabus).<\/h2>\n<p>&nbsp;<\/p>\n<h2>\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0To conduct your laboratory exercises, use the Laboratory Manual located under Course Content. Read the introduction and the directions for each exercise\/experiment carefully before completing the exercises\/experiments and answering the questions.<\/h2>\n<p>&nbsp;<\/p>\n<h2>\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Save your Lab 4\u00a0Answer Sheet\u00a0in the following format:\u00a0\u00a0LastName_Lab4 (e.g., Smith_Lab4).<\/h2>\n<p>&nbsp;<\/p>\n<h2>\u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0You should submit your document as a Word (.doc or .docx)\u00a0or\u00a0Rich Text Format (.rtf) file for best compatibility.<\/h2>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Pre-Lab Questions<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p>&nbsp;<\/p>\n<ol>\n<li>How could you test to see if an enzyme was completely saturated during an experiment?<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<ol>\n<li>List three conditions that would alter the activity of an enzyme. Be specific with your explanation.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<ol>\n<li>Take a look around your house and identify household products that work by means of an enzyme. Name the products, and indicate how you know they work with an enzyme.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>Experiment 1: Enzymes in Food<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Data Tables and Post-Lab Assessment<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>Table 1: Substance vs. Starch Presence<\/strong><\/p>\n<p>&nbsp;<\/p>\n<div>\n<table border=\"1\" width=\"584\">\n<tbody>\n<tr>\n<td colspan=\"3\"><strong>Table 1: Substance vs. Starch Presence<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"293\"><strong>Substance<\/strong><\/td>\n<td width=\"154\"><strong>Resulting Color<\/strong><\/td>\n<td width=\"137\"><strong>Presence of Starch?<\/strong><\/td>\n<\/tr>\n<tr>\n<td><strong>Positive Control: Ginger Root<\/strong><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td><strong>Negative Control:\u00a0<\/strong><strong><em>Student Must Select<\/em><\/strong><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td><strong>Food Product:<\/strong><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td><strong>Food Product:<\/strong><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td><strong>Saliva:<\/strong><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Post-Lab Questions<\/strong><\/p>\n<p>&nbsp;<\/p>\n<ol>\n<li>What were your controls for this experiment? What did they demonstrate? Why was saliva included in this experiment?<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<ol>\n<li>What is the function of amylase? What does amylase do to starch?<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<ol>\n<li>Which of the foods that you tested contained amylase? Which did not? What experimental evidence supports your claim?\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/li>\n<li>Saliva does not contain amylase until babies are two months old. How could this affect an infant\u2019s digestive requirements?\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/li>\n<li>There is another digestive enzyme (other than salivary amylase) that is secreted by the salivary glands. Research to determine what this enzyme is called. What substrate does it act on? Where in the body does it become activated, and why?<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<ol>\n<li>Digestive enzymes in the gut include proteases, which digest proteins. Why don\u2019t these enzymes digest the stomach and small intestine, which are partially composed of protein?<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Experiment 2: Effect of Temperature on Enzyme Activity<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>Data Tables and Post-Lab Assessment<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>Table 2: Balloon Circumference vs. Temperature<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<table border=\"1\" width=\"618\" align=\"left\">\n<tbody>\n<tr>\n<td colspan=\"5\" width=\"618\"><strong>Table 2: Balloon Circumference vs. Temperature<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"71\"><strong>Tube<\/strong><\/td>\n<td width=\"124\"><strong>Temperature (\u00b0C)<\/strong><\/td>\n<td width=\"141\"><strong>Uninflated Balloon Circumference (cm)<\/strong><\/td>\n<td width=\"133\"><strong>Final Balloon Circumference (cm)<\/strong><\/td>\n<td valign=\"top\" width=\"149\"><strong>Difference in Balloon Circumference (cm)<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"71\"><strong>1 &#8211; (Cold)<\/strong><\/td>\n<td width=\"124\">&nbsp;<\/td>\n<td rowspan=\"3\" width=\"141\">&nbsp;<\/td>\n<td width=\"133\">&nbsp;<\/td>\n<td valign=\"top\" width=\"149\">&nbsp;<\/td>\n<\/tr>\n<tr>\n<td width=\"71\"><strong>2 &#8211; (RT)<\/strong><\/td>\n<td width=\"124\">&nbsp;<\/td>\n<td width=\"133\">&nbsp;<\/td>\n<td valign=\"top\" width=\"149\">&nbsp;<\/td>\n<\/tr>\n<tr>\n<td width=\"71\"><strong>3 &#8211; (Hot)<\/strong><\/td>\n<td width=\"124\">&nbsp;<\/td>\n<td width=\"133\">&nbsp;<\/td>\n<td valign=\"top\" width=\"149\">&nbsp;<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>Post-Lab Questions<\/strong><\/p>\n<p>&nbsp;<\/p>\n<ol>\n<li>What reaction is being catalyzed in this experiment?\n<p>&nbsp;<\/li>\n<li>What is the enzyme in this experiment? What is the substrate?\n<p>&nbsp;<\/li>\n<li>What is the independent variable in this experiment? What is the dependent variable?\n<p>&nbsp;<\/li>\n<li>How does the temperature affect enzyme function? Use evidence from your data to support your answer.\n<p>&nbsp;<\/li>\n<li>Draw a graph of balloon diameter vs. temperature. What is the correlation?\n<p>&nbsp;<\/li>\n<li>Is there a negative control in this experiment? If yes, identify the control. If no, suggest how you could revise the experiment to include a negative control.\n<p>&nbsp;<\/li>\n<li>In general, how would an increase in substrate alter enzyme activity? Draw a graph to illustrate this relationship.\n<p>&nbsp;<\/li>\n<li>Design an experiment to determine the optimal temperature for enzyme function, complete with controls. Where would you find the enzymes for this experiment? What substrate would you use?<\/li>\n<\/ol>\n<p><center><a href=\"http:\/\/onlineclassesguru.com\/orders\/ordernow\"><img decoding=\"async\" src=\"https:\/\/encrypted-tbn0.gstatic.com\/images?q=tbn:ANd9GcTyj99p60XCLyLk1htB7-1neRt8-2QdnenNlQ&usqp=CAU\"target=\"_http:\/\/onlineclassesguru.com\/orders\/ordernow\"\/><\/center><p>","protected":false},"excerpt":{"rendered":"<p>INSTRUCTIONS: &nbsp; &nbsp; &nbsp; \u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0On your own and without assistance, complete this\u00a0Lab 4\u00a0Answer Sheet\u00a0electronically and submit it via the\u00a0Assignments Folder\u00a0by the date listed in the\u00a0Course Schedule\u00a0(under\u00a0Syllabus). &nbsp; \u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0To conduct your laboratory exercises, use the Laboratory Manual located under Course Content. Read the introduction and the directions for each exercise\/experiment carefully before completing the exercises\/experiments and&#8230;<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-1890","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v17.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Enzymes - onlineclassesguru<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/onlineclassesguru.com\/index.php\/2020\/10\/29\/enzymes-2\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Enzymes - onlineclassesguru\" \/>\n<meta property=\"og:description\" content=\"INSTRUCTIONS: &nbsp; &nbsp; &nbsp; \u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0On your own and without assistance, complete this\u00a0Lab 4\u00a0Answer Sheet\u00a0electronically and submit it via the\u00a0Assignments Folder\u00a0by the date listed in the\u00a0Course Schedule\u00a0(under\u00a0Syllabus). &nbsp; \u00b7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0To conduct your laboratory exercises, use the Laboratory Manual located under Course Content. 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